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aex buffer  (Cytiva Europe)


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    Structured Review

    Cytiva Europe aex buffer
    Aex Buffer, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 18577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aex+buffer/HEPES/pmc06592678-233-5-30
    Average 98 stars, based on 18577 article reviews
    aex buffer - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Purification:

    Article Title: tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay
    Article Snippet: The cleared supernatant was applied to a Ni 2+ affinity chromatography column (HisTrap FF 5 ml, GE Healthcare Life Sciences) and ct Trl1-LIG eluted with a 25–500 mM imidazole gradient (same as lysis buffer). .. LIG-containing fractions were dialysed against AEX buffer (20 mM HEPES/NaOH pH 8, 10 mM NaCl, 5 mM DTT) and further purified by anion exchange chromatography (HiTrap Q HP 5 ml, GE Healthcare Life Sciences) using a 10–1000 mM NaCl gradient in AEX buffer. .. Finally, the protein was applied to size-exclusion chromatography using a HiLoad 16/60 Superdex200 pg column (GE Healthcare Life Sciences) in SEC buffer (10 mM HEPES/NaOH pH 7.5, 100 mM NaCl, 5 mM DTT), concentrated and flash frozen in N 2 (l).

    Chromatography:

    Article Title: tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay
    Article Snippet: The cleared supernatant was applied to a Ni 2+ affinity chromatography column (HisTrap FF 5 ml, GE Healthcare Life Sciences) and ct Trl1-LIG eluted with a 25–500 mM imidazole gradient (same as lysis buffer). .. LIG-containing fractions were dialysed against AEX buffer (20 mM HEPES/NaOH pH 8, 10 mM NaCl, 5 mM DTT) and further purified by anion exchange chromatography (HiTrap Q HP 5 ml, GE Healthcare Life Sciences) using a 10–1000 mM NaCl gradient in AEX buffer. .. Finally, the protein was applied to size-exclusion chromatography using a HiLoad 16/60 Superdex200 pg column (GE Healthcare Life Sciences) in SEC buffer (10 mM HEPES/NaOH pH 7.5, 100 mM NaCl, 5 mM DTT), concentrated and flash frozen in N 2 (l).



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